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. Author manuscript; available in PMC: 2022 May 9.
Published in final edited form as: ACS Infect Dis. 2021 Apr 23;7(6):1795–1808. doi: 10.1021/acsinfecdis.1c00101

Figure 3.

Figure 3.

Membrane permeation and depolarization by the miniature LL-37 peptide C10-KR8d. (A) Effects of C10-KR8d, 17BIPHE2 and daptomycin on membrane integrity based on the fluorescence of a membrane non-permeable dye propidium iodide. S. aureus USA300 LAC treated with 12.5 μM of peptides and relative fluorescence intensity was recorded with time. 17BIPHE2 and daptomycin are used as positive controls, while rifamycin was included as a negative control for the assay. (B) Effects of peptides on membrane depolarization of the mid-log phase S. aureus USA300 re-suspended in PBS and glucose energized for 15 min at 37 °C. See Methods for further details. C10-KR8d, 17BIPHE2 and daptomycin (12.5 μM) were added to respective wells at post-dye equilibration pause cycle and the fluorescence was monitored for 40 minutes. The detergent Triton X-100 (0.1%) was included as a positive control.