Table 3.
Effects of salts, pH and human serum on peptide activity against Staphylococcus aureus USA300 LAC 1
| Peptide | Minimal inhibitory concentration (MIC, μM) | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 mM | 100 mM |
200 mM |
pH=6.8 | pH=7.4 | pH=8.0 | 5% Serum |
10% Serum |
|
| C10-KR8 | 3.1 | 3.1 | 3.1 | 3.1-6.2 | 3.1 | 1.6 | >25 | >25 |
| C10-KR8d | 3.1 | 3.1 | 3.1 | 3.1 | 3.1 | 1.6 | 3.1-6.2 | 6.2 |
| C8-KR10 | 6.2 | 6.2 | 6.2 | 12.5 | 6.2 | 3.1 | >25 | >25 |
| C8-KR10d | 3.1 | 3.1 | 3.1 | 6.2 | 3.1 | 1.6 | 3.1 | 6.2 |
1
The MIC was normally obtained in 100% TSB at pH 7.4 (indicated by 0 mM in the table). The condition of the TSB was then altered by adjusting pH, adding NaCl or human serum.