Table 4.
Antimicrobial susceptibility of the transposon mutants of S. aureus USA300 to C10-KR8d, a miniature lipoLL-37 peptide 1
| Gene ID | Name | Potential function | MIC (μM) |
Susceptible? |
|---|---|---|---|---|
| SAUSA300 | JE2 | Wild type (WT) | 3.1 | Reference |
| SAUSA300_1962 | phiPVL ORF39-like protein | 1.6-3.1 | Yes | |
| SAUSA300_1255 | mprF | Oxacillin resistance-related FmtC protein | 1.6 | Yes |
| SAUSA300_0829 | lipA | Lipoyl synthase | 3.1 | No |
| SAUSA300_1036 | RNA methyltransferase | 1.6-3.1 | Yes | |
| SAUSA300_1465 | 2-oxoisovalerate dehydrogenase, E1 component, beta subunit | 3.1 | No | |
| SAUSA300_0186 | argC | N-acetyl-gamma-glutamyl-phosphate reductase | 3.1 | No |
| SAUSA300_0988 | trkA | Potassium uptake protein | 1.6 | Yes |
| SAUSA300_1089 | lspA | lipoprotein signal peptidase | 1.6 | Yes |
| SAUSA300_1515 | ABC transporter permease | 1.6-3.1 | Yes | |
| SAUSA300_1967 | conserved hypothetical phage protein | 3.1 | No | |
| SAUSA300_1228 | thrB | homoserine kinase | 1.6-3.1 | Yes |
| SAUSA300_0394 | FAD/NAD(P)-binding Rossmann fold superfamily protein | 3.1 | No | |
| SAUSA300_0646 | graS | Sensor histidine kinase | 1.6 | Yes |
| SAUSA300_0645 | graR | DNA-binding response regulator | 1.6 | Yes |
| SAUSA300_1865 | vraR | DNA-binding response regulator | 1.6 | Yes |
| SAUSA300_0647 | vraF | ABC transporter ATP-binding protein | 1.6-3.1 | Yes |
| SAUSA300_1336 | Hypothetical protein | 3.1 | No | |
| SAUSA300_1898 | Conserved hypothetical protein | 3.1 | No | |
| SAUSA300_1037 | pheS | Phenylalanyl-tRNA synthetase subunit alpha | 1.6 | Yes |
| SAUSA300_1503 | Putative competence protein ComGB | 3.1 | No | |
| SAUSA300_1867 | Conserved hypothetical protein | 3.1 | No | |
| SAUSA300_1097 | pyrF | orotidine 5'-phosphate decarboxylase | 3.1 | No |
1
Mutants found previously in response to human cathelicidin LL-37 and its major antimicrobial peptides GF-17 and 17BIPHE2 [49]. No = no MIC changes of C10-KR8d against the WT and the mutant S. aureus strains measured in 50% TSB.