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. Author manuscript; available in PMC: 2022 Sep 30.
Published in final edited form as: Sci Transl Med. 2022 Mar 30;14(638):eabl8574. doi: 10.1126/scitranslmed.abl8574

Figure 5. Combined IL-1 and TNF blockade protects the developing lung from inflammatory injury.

Figure 5.

A-D) Experimental design and histological analysis of LPS-induced lung injury following combination blockade. E-J) Quantification of lung injury score (E), septal number (F), and lung morphometry (G-J) show near normalization compared to control and significant improvement compared to LPS. Quantification data reproduced from Figure 2 for comparison. K-S) Combination blockade prevents disruption of AT1/AC interactions. T-BB) Maintenance of differentiated epithelial cells in alveoli after combination blockade. CC-DD) Quantification of EDNRB+ surface volume (CC) and HOPX+ nuclei (DD) following combination blockade. Concordant with morphometry, AT1 cell numbers and EDNRB+ volume improve with blockade. Quantification data reproduced from Figure 1 for control and LPS comparison. * = p <0.05, ** = p <0.01, *** = p <0.001, **** = p < 0.0001 by Kruskal-Wallace test. Scale bars = 50μm.