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. 2022 May 5;221(7):e202008116. doi: 10.1083/jcb.202008116

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© 2022 Huang and Winklbauer

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Figure 3. — Diffuse Pk1 overlaps with cortical F-actin. (A) PCM cell labeled with mb-RFP and Pk1-venus. Scale bar, 10 μm. (B) WT and SMIFH2- and CK666-treated PCM cells labeled with LifeAct-Ruby and Pk1-venus. Arrows, small Pk1 puncta. Y, yolk platelets. Scale bars, 10 μm. (B′) Line plots of LifeAct-Ruby and Pk1-venus intensities at dotted lines in B. (C) Widths of F-actin (LifeAct-Ruby) and diffuse Pk1-venus zones in WT (F-actin, n = 32; Pk1, n = 36) and SMIFH2-treated (F-actin, n = 66; Pk1, n = 68) cells. F-actin and Pk1-venus became dispersed in CK666-treated cells, and the widths could not be determined (N/D). n, number of cells. Means ± SD are shown. ****, P ≤ 0.0001 in a two-tailed Student’s t test. Data distribution was assumed to be normal but was not formally tested.