Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 5;221(7):e202008116. doi: 10.1083/jcb.202008116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Huang and Winklbauer

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Pk1-Dvl2-CKII regulates F-actin dynamics at the cell membrane. (A and A′) PCM cells labeled with LifeAct-Ruby and Pk1-venus showing membrane blebs. In WT (A), a bleb forms near the bottom and retracts near the top, creating a circular movement (arrow). In the TBB-treated cell (A′), the bleb forms a wide crescent. Right, enlargements of boxed areas. Scale bars, 10 μm. (A″) Intensity plots of F-actin and Pk1 at dotted lines in Aand A′. Black arrows, positions of membrane-attached cortex; gray arrows, cell body surface. (B and B′) Time-series of LifeAct-GFP–labeled WT (B; Video 3) and TBB-treated (B′; Video 4) cells. Time in minutes. TBB was added at time 0:00. Yellow arrows, F-actin assembly at detached membrane; white arrows, newly assembled actin filaments in bleb. Scale bars, 10 μm. (C) Live PCM cells labeled with LifeAct-GFP and ER-Tracker Red. Inner cortex layer at bleb (arrow) conforms to the ER domain. Scale bar, 10 μm. (D) Live PCM cells labeled with Pk1-venus and ER-Tracker Red. Diffuse Pk1 appears enriched at cortex and in blebs but also fills cytoplasm; Y, yolk platelets. Scale bar, 10 μm. (E and E′) Live PCM cells labeled with blue dextran (BDX) and mb-RFP; Y, yolk platelets. Scale bars, 10 μm. (F) Live PCM cells labeled with Pk1-venus, BDX, and mb-RFP. Scale bar, 10 μm. (F′) Average intensity line plot of Pk1 and BDX across the cell periphery (cell interior to the left; n = 24). n, number of cells.