Table 1.

Equilibrium and kinetic data for α1, α0α1, β16, β16β17, and the tetramer domain

	mD–N (kcal mol−1 M−1)	[Den]50% (M)	ΔGD–N (kcal mol−1)	$k_{\text{f}}^{{\text{H}}_2\text{O}}$ (s−1)	$k_{\text{u}}^{{\text{H}}_2\text{O}}\left(k_{-}^{{\text{H}}_2\text{O}}\right)$ (s−1)	$m_{k_{\mathfrak{f}}}$ (M−1)	$m_{k_{\text{u}}}$ (mk−) (M−1)
α1a,c	1.38 (±0.01)	3.47 (±0.01)	4.78 (±0.03)	—	—	—	—
α1 in α0α1a	1.73 (±0.03)	3.75 (±0.01)	6.49 (±0.03)	1500 (±400)	0.08 (±0.06)	1.3 (±0.1)	1.4 (±0.2)
β16	3.44 (±0.03)	4.19 (±0.01)	14.4 (±0.1)	5200 (±800)	3 × 10−8 (±2 × 10−8)	3.22 (±0.06)	2.7 (±0.1)
β16 in β16β17	3.3 (±0.1)	4.30 (±0.01)	14.2 (±0.1)	4000 (±3000)	1.7 × 10−7 (±1.5 × 10−7)	3.0 (±0.2)	2.7 (±0.1)
R15a,d	1.8 (±0.1)	3.7 (±0.1)	6.8 (±0.2)	25,000 (±8000)	2.0 (±0.5)	1.85 (±0.08)	1.00 (±0.03)
R16a,d	1.9 (±0.1)	3.6 (±0.1)	6.4 (±0.2)	136 (±4)	1.11 × 10−2 (±6 × 10−4)	2.08 (±0.02)	0.885 (±0.008)
R17a,d	2.0 (±0.1)	3.0 (±0.1)	6.0 (±0.2)	27.9 (±1.2)	5.7 × 10−4 (±4 × 10−5)	2.29 (±0.03)	1.46 (±0.01)
Wild-type tetramere	—	—	—	—	2.5 × 10−4 (±3 × 10−5)	—	1.44 (±0.07)

^a[Den]_50%, m_{D–N, $m_{k_{\text{f}}}$}, $m_{k_{\text{u}}}$, and m_k−, reported in molar units of urea.

^bChevron not fitted.

^c[Den]_50%, m_{D–N, $m_{k_{\text{f}}}$}, and $m_{k_{\text{u}}}$ reported in molar units of GdmCl.

^dData from Ref. [48].

^e$k_{-}^{{\text{H}}_2\text{O}}$ and m_k− reported in lieu of $k_{\text{u}}^{{\text{H}}_2\text{O}}$ and $m_{k_{\text{u}}}$.