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(A and B) Examples of blood nucleated cells from patients with metastatic uveal melanoma that were positive for CD271 and negative for CD45 (arrows) and (C) virtual confocal imaged slice cells of cells positive for CD271 and Melan-A (arrow). Nuclei are visualized with DAPI (blue color). (D) Example of the formation of non-adherent spheres by CD271+ circulating cells from a patient with metastatic uveal melanoma cultured for 4 days in stem-cell supportive media.
