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. 2022 May 9;12:7570. doi: 10.1038/s41598-022-11230-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Visualization and sizing of ALF55 and ALFQ. ALF55, ALFQ, and ALFQsuv samples were visualized by cryo-electron microscopy (cryo-EM) and size distributions were measured by dynamic light scattering analysis (DLS). (a) Shows EM of small unilamellar vesicles in ALF55 (left) and their narrow size distribution (right). (b) Shows EM of both small and large vesicles in ALFQ (left) and a wider size distribution of the small and some large vesicles (right). However, DLS is not suitable to capture the range of the large size particles in ALFQ. (c) Shows the narrow size distribution of the vesicles in ALFQsuv.