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. 2022 Apr 25;13:798751. doi: 10.3389/fpls.2022.798751

TABLE 1.

Details of selected genes and primers designed by using the NCBI primer-BLAST tool for the quantitative real-time PCR (qRT-PCR) analysis of the plants treated with 15 mg/L of Zn NPs.

Plants Gene/Accession No 5′ Primer 3′ Primer
Faisal canola BnaC09g00220D/PsaD TGCTTGAATTC
CTAAGTTTGC
AATATGCCCA
TTCCCATCAA
BnaC03g44500D ATTGTCAAGG
CTTCTGCTTA
AGAATGAAAT
GCTCTCACCT
BnaA01g14450D/FSD1 GGTCCACTAA
GGAAGAAACA
TCCTAGCTTCG
GCTATATCA
Shiralee BnaC09g00220D/PsaD TTGTCGTTG
TCGTTAAAACC
TTGCTTAAAC
CATGCTACGA
BnaA04g15980D GAGTTGATTG
CTGTTGGAAG
CCCATGTCCT
GCAAATTAAC
BnaAnng33440D TTGGTTTGTC
CATGTTGTGA
CCGAGAATGAG
TAACGAGTA

Three biological replicates were used for gene expression.