Fig. 5. Raman spectra of bacterial cells before (black) and after (red) heat treatment. E. coli WT cells were grown for 8 h in shaking condition at 37 °C and 160 rpm. Bacteria were heat treated at 85 °C for 15 minutes. The shift in the peak maxima of Amide I is shown by the second derivative spectra. All spectra in the top panel were corrected for cosmic rays, background subtracted and vector normalised prior to analysis.
