Bioconversion of FAL during fermentation with B. coagulans NL01a.
| Entry | Initial FAL [mM] | t [h] | FAL [mM] | FOL [mM] | t [h] | FAL [mM] | FOL [mM] | t [h] | FAL [mM] | FOL [mM] | t [h] | FAL [mM] | FOL [mM] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 14.7 ± 0.1 | 12 | 1.8 ± 0.23 | 9.1 ± 0.04 | 24 | 0.0 | 11.1 ± 0.1 | 36 | 0.0 | 10.8 ± 0.1 | 60 | 0.0 | 10.6 ± 0.2 |
| 2 | 22.2 ± 0.1 | 12 | 3.2 ± 0.1 | 18.0 ± 0.2 | 24 | 0.0 | 19.7 ± 0.1 | 36 | 0.0 | 19.2 ± 0.1 | 60 | 0.0 | 19.1 ± 0.1 |
| 3 | 33.3 ± 0.1 | 12 | 31.5 ± 0.1 | 1.5 ± 0.1 | 24 | 4.1 ± 0.1 | 25.9 ± 0.1 | 36 | 0.0 | 27.2 ± 0.2 | 60 | 0.0 | 27.1 ± 0.2 |
| 4 | 40.6 ± 0.1 | 12 | 37.5 ± 0.1 | 1.4 ± 0.1 | 24 | 14.1 ± 0.3 | 24.0 ± 0.2 | 36 | 1.6 ± 0.1 | 35.9 ± 0.0 | 60 | 0.0 | 35.6 ± 0.2 |
| 5 | 52.1 ± 0.0 | 12 | 53.6 ± 0.1 | 0.0 | 24 | 53.6 ± 0.1 | 0.0 | 36 | 52.0 ± 0.2 | 0.0 | 60 | 49.8 ± 0.1 | 0.0 |
a
Reaction conditions: 10–52 mM FAL were added into 50 mL culture medium, 150 rpm, 50 °C; after microbial cells were anaerobic incubated for 60 h under the above conditions.