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. 2022 Feb 28;17(10):2238–2246. doi: 10.4103/1673-5374.336871

Figure 3.

Figure 3

EVs promote M2 polarization in BV-2 cells after OGD.

(A) qRT-PCR analysis of pro- and anti-inflammatory cytokine mRNA expression in BV-2 cells after 1-, 3-, or 5-hour OGD followed by 24-hour reoxygenation. n = 6 per group. (B) qRT-PCR analysis of the mRNA levels of IL-1β, iNOS, TNFα, IL-10, TGF-β, and CD206 in the presence or absence of MSCs-EVs. Cells had undergone 3-hour OGD followed by 24-hour reoxygenation. Cells incubated under standard cell culture conditions formed the “control” group. Graphs show the mean ± SD. All of the experiments were carried out on six separate samples. *P < 0.05, **P < 0.01, ***P < 0.001 (independent samples t-test) in A; *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance with Bonferroni correction) in B. EV: Extracellular vesicle; IL: interleukin; iNOS: inducible nitric oxide synthase; MSC: mesenchymal stromal cell; OGD oxygen-glucose deprivation; qRT-PCR: quantitative reverse transcription PCR; TGF-β: transforming growth factor-β; TNF-α: tumor necrosis factor α.