Fig. 4. Evaluation of disorder predisposition (A) and disorder-based interactivity (B) of the CHIKV TF protein. Intrinsic disorder propensity was evaluated by four predictors of the PONDR family: PONDR® VLXT (black line), PONDR® VL3 (red line), PONDR® VSL2B (green line) and PONDR FIT (pink line). Light pink shadow around PONDR FIT curve shows the error distribution. Blue dashed line represents the mean disorder propensity calculated by averaging the outputs of individual predictors. The presence of MoRF regions was evaluated by ANCHOR (blue line) and MoRFchibi (red line). The threshold for MoRF predictions by ANCHOR and MoRFchibi are 0.5 and 0.725. These thresholds are shown as dashed red and blue lines, respectively. Positions of MoRFs predicted by ANCHOR and MoRFchibi are shown by cyan and light pink bars, respectively.