Table 1.
Strains and plasmids used in this study.
| Strain/Plasmid | Description | Source or Reference |
|---|---|---|
| Strain | ||
| DH5α competent cell | A strain for screening or storing (recombinant) plasmids | Purchased from TAKARA |
| DH5α/pWH1266 | A strain with a pWH1266 plasmid | Lab stock |
| ATCC 19606 | A standard A. baumannii strain, wild-type | Lab stock |
| 19,606/p-RS | An overexpressed strain of BIT33_RS14560, containing a pWH1266 plasmid whose TetR was deleted and replaced by BIT33_RS14560 | This study |
| 19,606/p | A control strain, containing a pWH1266△(TetR) plasmid but without the insertion by BIT33_RS14560 | This study |
| XAb53 | An XDR-Ab isolate, wild-type | This study |
| XAb53/p-telRs-RS | An overexpressed XDR-Ab strain of BIT33_RS14560, containing a pWH1266△(TetR) plasmid inserted by BIT33_RS14560 and its AmpR was replaced by TelR | This study |
| XAb53/p-telRs | A control strain, containing a pWH1266△(TetR) plasmid without the insertion by BIT33_RS14560, but its AmpR was also replaced by TelR | This study |
| XAb50 | An XDR-Ab isolate, wild-type | This study |
| XAb50/p-telRs-RS | An overexpressed XDR-Ab strain of BIT33_RS14560, containing a pWH1266△(TetR) plasmid inserted by BIT33_RS14560 and its AmpR was replaced by TelR | This study |
| XAb50/p-telRs | A control strain, containing a pWH1266△(TetR) plasmid without the insertion by BIT33_RS14560, but its AmpR was also replaced by TelR | This study |
| Plasmid | ||
| pWH1266 | A shuttle plasmid with AmpR and TetR | Extracted from DH5α/pWH1266 strain |
| pMo130-telR | A suiside plasmid with TelR, NeoR, and/or KanR | Purchased from Wonder Biotech |
XDR-Ab isolates refer to clinic isolates with resistance to at least one agent in all but two or fewer antimicrobial categories (Magiorakos et al., 2012). The two XDR-Ab isolates were identified by antibiotic susceptibility testing. TetR, tetracycline resistance; XDR-Ab, extensively drug-resistant A. baumannii; AmpR, ampicillin resistance; TelR, tellurite resistance; NeoR, neomycin resistance; and KanR, kanamycin resistance.