FIG. 2.

Variation in polarization of DPH (top panel) and TMA-DPH (bottom panel) fluorescence incorporated in liposomes made of cholesterol, phosphatidylcholine, phosphatidylinositol, and sphingomyelin (33.3, 24.2, 18.3, and 24.2%). Vesicles (300 μM) were incubated with butenafine at increasing concentrations. ■, butenafine solvent (control); Δ, butenafine (25 μM; 8 μg/ml); ▾, butenafine (250 μM; 80 μg/ml); ◊, butenafine (500 μM; 160 μg/ml); ●, butenafine (1,000 μM; 320 μg/ml). Each point is the mean value of four independent experiments; standard deviations are not shown for the sake of clarity.