Fig. 5.

Systemic administration of the GW803430 (MCHR1 antagonist) to mice increases cilia length. a Schematic representation of systemic administration of GW803430 or vehicle for 7 consecutive days in adult mice. Diagram was created with BioRender.com webpage. b, e, h, k Immunostaining of ADCY3 labeled in red fluorescence in the b PFC, e CA1, h CPu, and k NAc in animals administered with GW803430 or vehicle (scale bar = 10 μm). c, f, I, l Quantification of the length of ADCY3 + primary cilia in the c PFC, f CA1, i CPu, and l NAc in animals administered with GW803430 or vehicle, *P < 0.05, **P < 0.01, and ***P < 0.001. d, g, j, m Cilia were grouped by length (μm) and plotted against the number of cells (%) in the d PFC, g CA1, j CPu, and m NAc