Cilia length is increased in MCHR1 knockout animals. Immunostaining of ADCY3 labeled in red fluorescence and counterstained with DAPI (blue) in the a PFC, d CA1, g CPu, and j NAc in MCHR1 KO or WT mice (scale bar = 10 μm, magnification = 63 ×. b, e, h, k Quantification of the length of ADCY3+ primary cilia (μm) in the b PFC, e CA1, h CPu (CPu), and k NAc in MCHR1 KO or WT mice, **P < 0.01 and ***P < 0.001. c, f, I, l Cilia were grouped by length (μm) and plotted against the number of cells (%) in the c PFC, f CA1, i CPu, and l NAc