Table II.

Primers, annealing PCR and amplicon sizes of PCR products corresponding to the pathogenic variants and variants resulted deleterious by in silico analysis.

Gene variants	Primer sequences (5′→3′)	TM (°C)	Amplicon (bp)	Exon
MLH1 c.350C>T	F: GTGACCCAGCAGTGAGTTTT	58	245	4
	R: AGCCTCACTTTTACCCTCTCT
MSH6 c.3311_3312del	F: AGCCTCACTTTTACCCTCTCT	58	398	5
	R: TGGCTGACTTTTATGTAACTGTG
MSH6 c.892C>T	F: TGGTGGCTCTGATGTGGAAT	59	206	4
	R: TTGCTTGTTTGGTGGCTGAG
ATM c.3802delG	F: TGCTACTGAACAAGGTCCCA	59	448	26
	R: CTCTCTTTGCTGTGCCATCC
MLH1 c.376T>A	F: GTGACCCAGCAGTGAGTTTT	58	245	4
	R: ACGTACTCAAGATCTCTGCCA
EPCAM c.332A>G	F: TGATGAAGGCAGAAATGAATGG	58	250	3
	R: ACAAGTAGTATAGGCAGCCCC
MSH3 c.1778G>A	F: CCTGGGCATTAGAGTGGGAA	58	264	13
	R: TGTCCTCAAGCTGAAGAACAC
MLH3 c.470T>C	F: TATGGTTTCCGAGGAGAGGC	59	232	2
	R: CCAGTCTAGGGTCCATGCAT
MLH3 c.3440A>T	F: TCAGTTTGTGCAGAAAGAGGT	58	250	3
	R: GAGTTAGGTGGTACGATGTGT
ATM c.7475T>G	F: AGGAAGGTGTGTGAATTGCA	58	465	50
	R: CCTGACATCAAGGGGCTTATG
ATM c.1178G>T	F: GGCAACAACAGCGAAACTCT	58	396	9
	R: TGTCATGGCAATCACATATCCC
ATM c.8734A>G	F: ATTAGCTGTCAAACCTCCTAACT	58	221	60
	R: TGCCCAGCCCATGTAATTTT
CHEK2 c.911T>C	F: TGTCTTCTGTCCAAGTGCGT	59	245	9
	R: GGTCCCTCGATTTCTGCCTA
PMS2 c.1004A>G	F: AAAGTGAATTTGGCTGGGCG	59	498	10
	R: TGGCTGCTGACTGACATTTAGCTTG
PMS2 c.2249G>A	F: TCTCAGGAAGTTTTGTGACACT	59	295	13
	R: CACCCAGCCGCTATAGTTCT
PMS2 c.1253C>T	F: GACCCTCTTCTCCGTCCAC	58	491	11
	R: GAGAGTCCACATGTTCCTGC
MLH1 c.589-9_589-6delGTTT	F: GTTTGCTGGTGGAGATAAGGT	58	392	8
	R: ACGCCACAGAATCTAGGAGA
MRE11 c.1783+7A>G	F: ACTTTCTCCTTCTTCTCCCTCT	59	410	15
	R: TGTCAGAACTGCCTTAAAGACTG
CDH1 c.585A>C	F: TTCTCTGGGAGGGATTTGGC	59	293	5
	R: CCCGGTGTCAACAAGCTTC
CDH1 c.344C>T	F: GAAGATTGCACCGGTCGAC	58	250	3
	R: CAACAGCGAACTTCTCAGAAAA
NF1 c.4445T>C	F: CTGGGTGTATCTGGTGTTGAAAA	58	486	34
	R: GGATCTATAACAATCTGCAAGCC
CHEK2 c.688G>T	F: CTTGAAGTGGACCCAGGAGT	58	242	6
	R: TGGGAAGTTATGAAGACGTGTT

F, forward; R, reverse.