FIG. 2.

Expression of C. albicans CDR1 mRNA and Cdr1p in S. cerevisiae AD1002. (A) Total RNA (20 μg) from parental AD1-8u⁻ cells, AD1-8u⁻ cells transformed with shuttle plasmid pSK-PDR5PPUS, or AD1002 cells was hybridized with a mixture of [α-³²P] dCTP-labeled C. albicans CDR1 and S. cerevisiae PMA1 (control) probes. The lower part of panel A shows a portion of the ethidium bromide-stained agarose gel before vacuum blotting. (B) Plasma membrane proteins separated through an 8% polyacrylamide gel and stained with Coomassie blue. Lane M, molecular mass markers. (C) Plasma membrane proteins separated through an 8% polyacrylamide gel, electroblotted onto nitrocellulose, and incubated with anti-C. albicans Cdr1p antibodies. Antibodies were detected with horseradish peroxidase-labeled anti-immunoglobulin G.