Azide-dependent fluconazole accumulation by S. cerevisiae AD1002. [3H]fluconazole accumulation was measured in the presence (open symbols) or absence (closed symbols) of sodium azide (20 mM). The strains used were AD1-8u− (■, □), AD/pSK-PDR5PPUS (⧫, ◊), and AD1002 (●, ○). Results are the means ± standard deviations of six separate determinations with two batches of cells.