FIG. 5.

Energy-dependent Cdr1p-mediated efflux of rhodamine 6G from yeast cells. Deenergized cells were preloaded with rhodamine 6G as described in Materials and Methods. The efflux of rhodamine was followed by direct measurement of the fluorescence in cell supernatants following the addition of glucose (2 mM) to suspensions of S. cerevisiae AD1002 (●) or AD1-8u⁻ (■) cells. The fluorescence in the supernatants of AD1002 cells incubated in the absence of added glucose is also shown (○). The results are the means ± standard deviations of triplicate determinations from a representative experiment which was undertaken three times.