TABLE 2.
Oligomycin-sensitive NTPase activities in plasma membrane fractions of parental strain S. cerevisiae AD1-8u− and S. cerevisiae AD1002 expressing Cdr1p
| S. cerevisiae strain | Oligomycin-sensitive NTPase activity (nmol of Pi min−1 mg of protein−1)a
|
|||||
|---|---|---|---|---|---|---|
| UTPase
|
CTPase
|
GTPase
|
||||
| pH 6.5 | pH 7.5 | pH 6.5 | pH 7.5 | pH 6.5 | pH 7.5 | |
| AD1-8u− | 1 | 0 | 1 | 0 | 0 | 0 |
| AD1002 | 16 | 30 | 24 | 40 | 27 | 46 |
a
NTPase activities were determined in assays similar to those that provided the data presented in Fig. 3. Values represent the differences in NTPase activities measured in the presence and absence of 20 μM oligomycin. The results are the means of two experiments, which did not vary more than 10%.