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. 2022 Apr 25;13:864631. doi: 10.3389/fendo.2022.864631

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Copyright © 2022 Xu, Tian, Kang, Yuan, Liu, Li, Liu and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockout of nur77 in zebrafish by Crispr/Cas9. (A) Schematic representations of Crispr-Cas9 targets and mutant alleles. nur77 consists of 6 exons (filled box). The sgRNA target exon 1, and the sequences of the target region were aligned to the selected allele. The target site was in red font, and the PAM site was in blue font. The obtained nur77^−/− zebrafish mutant lacked 13 bp bases, causing protein translation to stop prematurely. (B) The validation of the expression level of nur77 by qRT-PCR analysis (n = 3); ^* p < 0.05 by t-test. (C) The morphology of WT and nur77^−/− in developmental stages of 12, 24, 48, 72, and 144 hpf. Scale bars indicate 750 μm.