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. 2022 Apr 25;13:864631. doi: 10.3389/fendo.2022.864631

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Copyright © 2022 Xu, Tian, Kang, Yuan, Liu, Li, Liu and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nur77 regulates amino acid metabolism in zebrafish larvae. (A) Heatmaps of transcripts in amino acid metabolism enrichment. Colors represent high (red), low (blue), or average (white) expression values based on Z-score-normalized fragments per kilobase per million mapped read (FPKM) values for each gene. The Z-score indicators are shown under each map. The fold change (log2) is shown on the right. (B) The validation of the expression levels of differentially expressed genes by qRT-PCR analysis in the categories of amino acid metabolism (n = 3). (C) Disturbed valine, leucine, and isoleucine degradation. Blocks represent transcript-encoded enzymes. Green blocks represent downregulated genes, and black blocks represent unchanged genes. (D) Amino acids compositions in WT and nur77 ^−/− embryos. Results are represented as means with standard errors (n = 3); ^* p < 0.05; ^** p < 0.01; ***p < 0.001, ****p < 0.0001 by t-test.