Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 25;13:883743. doi: 10.3389/fimmu.2022.883743

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Serrano, Luque, Mitjavila, Blom, Rodríguez de Córdoba, Vega, Torras and Aran

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

PRP6-HO7 prevents pro-inflammatory intracellular (endosomal) TLR activation of Mo-DCs. Human Mo-DCs were incubated throughout their differentiation process with C4BP(β+), C4BP(β-) (both at 12 nM) and PRP6-HO7 (32 nM). DC maturation was achieved by TLR agonist treatment: Poly I:C HMW (TLR3), Poly I:C LMW (TLR3), Gardiquimod (TLR7), ssRNA40/LyoVec (TLR8), E.coli ssDNA/LyoVec (TLR9). Cells were then collected, washed, and analyzed by flow cytometry for cell surface expression of CD83, CD86, CD80, CD40 and HLA-DR. MFI, median fluorescence intensity for the different surface markers. iDC, untreated, immature DCs; mDC, untreated, TLR-matured DCs. The results shown are the mean ± SD from 4-10 independent donors (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 compared with mDC).