Figure 8.

PRP6-HO7 displays immunomodulatory activity in differentiating Mo-DCs and Mo-macrophages from active autoimmune SLE patients. Human Mo-DCs from active SLE patients (cohort 1) were incubated throughout their differentiation process with C4BP(β+) and C4BP(β-) (both at 12 nM), and with PRP6-HO7 at 32 nM. DC maturation was achieved by LPS (A) or gardiquimod (Gdq) (B) treatment. Cells were then collected, washed, and analyzed by flow cytometry for cell surface expression of the activation marker CD83, the co-stimulatory molecules CD86, CD80 and CD40, and HLA-DR. MFI, median fluorescence intensity for the different surface markers. The concentrations of IL-12p70, and TNF-α were analyzed in the respective cell supernatants by ELISA. The results shown are the mean ± SD from 10 (LPS) or 11 (Gdq) independent donors. iDC, untreated, immature DCs; mDC, untreated, LPS-matured DCs (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared with mDC).