TABLE 1.
Strains and plasmids used in this study.
| Name | Relevant Characteristics | References |
|---|---|---|
| Strains | ||
| E.coli DH5α | F− recA endA1Φ80dlacZ△M15hsdR17(rk -mk +)λ− | Invitrogen |
| E.coli BL21 (DE3) | F- ompT hsdSB (rB – mB - ) gal dcm rne131 λ(DE3) | Invitrogen |
| E.coli JM109 (DE3) | endal glnv44 thi−1 relal gyra96 recal mcrB+△(lac-proAB) e14−[F, traD36 proAB+ lacZ △M15] hsdr17(rk− mk+) λ(DE3) | Invitrogen |
| E.coli Rosstta (DE3) | F-ompT hsdSB(rB-mB-)gal dcm(DE3) | Invitrogen |
| E.coli MG1655 (DE3) | F- λ- ilvg- rfb-50 rph-1λ(DE3) | Invitrogen |
| srain 01 | E.coli. BL21(DE3)/pACYCDuet-2-cymA | This study |
| srain 02 | E.coli. BL21(DE3)/pET28a(+)-cymA | This study |
| srain 03 | E.coli. BL21(DE3)/pRSFDuet-1-cymA | This study |
| srain 04 | E.coli. BL21(DE3)/(pRSFDuet-1-cymA and pACYCDuet-2-lac-alkL) | This study |
| srain 05 | E.coli. BL21(DE3)/(pRSFDuet-1-cymA and pACYCDuet-2-trc-alkL) | This study |
| srain 06 | E.coli. BL21(DE3)/(pRSFDuet-1-cymA and pACYCDuet-2-alkL) | This study |
| srain 07 | E.coli. JM109 (DE3)/(pRSFDuet-1-cymA and pACYCDuet-2-lac-alkL) | This study |
| srain 08 | E.coli. Rosstta (DE3)/(pRSFDuet-1-cymA and pACYCDuet-2-lac-alkL) | This study |
| srain 09 | E.coli. MG1655 (DE3)/(pRSFDuet-1-cymA and pACYCDuet-2-lac-alkL) | This study |
| srain 10 | E.coli. MG1655 (DE3)/(pRSFDuet-1-cymA-fdh and pACYCDuet-2-lac-alkL) | This study |
| Plasmids | ||
| pRSFDuet-1 | double T7 promoters, RSF ori, KanR | Novagen |
| pET28a(+) | KanroripBR322lacIqT7p | Novagen |
| pACYCDuet-1 | Cmr p15A lacI T7lac | Novagen |
| pACYCDet-2 | Ampr p15A lacI T7lac | This study |
| pACYCDet-2-cymA | pACYCDet-2 carrying cymAa and cymAb from Pseudomonas putida | This study |
| pET28a(+)-cymA | pET28a(+) carrying cymAa and cymAb from Pseudomonas putida | This study |
| pRSFDuet-1-cymA | pRSFDuet-1 carrying cymAa and cymAb from Pseudomonas putida | This study |
| pACYCDet-2-lac-alkL | pACYCDuet-2 carrying alkL from P. putida GPo1, the alkL gene with lac promoter | This study |
| pACYCDet-2-trc-alkL | pACYCDuet-2 carrying alkL from P. putida GPo1, the alkL gene with trc promoter | This study |
| pACYCDet-2-alkL | pACYCDuet-2 carrying alkL from P. putida GPo1, the alkL gene withT7 promoter | This study |
| pRSFDuet-1-cymA-fdh | pRSFDuet-1 carrying cymAa and cymAb from Pseudomonas putida, fdh from Candida boidinii | This study |