Chemical composition of Eagle's Minimum Essential Medium (Eagles' MEM “Nissui” 1).

Components	Concentration (mg L−1)	Components	Concentration (mg L−1)
NaCl	6800	l-Phenylalanine	32
NaHCO3	2200	l-Cystine·HCl·H2O	31.4
KCl	400	l-Methionine	15
CaCl2	200	l-Tryptophan	10
NaH2PO4	115	Sodium succinate·6H2O	100
MgSO4	93.5	Succinic acid	75
d-Glucose	1000	i-Inositol	2
Phenol red	6	Choline bitartrate	1.8
l-Glutamine	292	d-Ca pantothenate	1
l-Arginine·HCl	126	Folic acid	1
l-Lysine·HCl	73	Pyriodoxal HCl	1
l-Leucine	52	Niacinamide	1
l-Isoleucine	52	Thiamine HCl	1
l-Threonine	48	Riboflavin	0.1
l-Valine	46	Biotin	0.02
l-Histidine·HCl·H2O	42	Kanamycin	60 (titer)
l-Tyrosine	36