Table 1. pre-miRNA obtained from VMir and further validated using imiCRNA and RNAstructure.

Sr.no#	miRNA name	Orientation	Sequence	Genome location	Size, nt	MFE, kcal/mol
1	H5N1V-MD96	Reverse	GGCAGCGGAAGCCATGGAGGTTGCTAGTCAGGCTAGGCAGATGGTGCAGGCAATGAGGACAATTGGGACTCATCCTAGCTCCAGTGCCGGTCTGAAAGATAATCTTCTTGAAAATTTGCAGGCC	12576–12699	124	-46.3
2	H5N1V-MR124	Reverse	AGTGGGTTGGTGGTAGTTGCCATCTGTCTGTGAGACCGATGCTGTGAATCTGCAATCTGCTCACAAGTGGCACACACTAGGCCAAAAGCCACT	12405–12497	93	-30.7
3	H5N1V-MR46	Reverse	TGGGTATCTGTAGTCCTTAATGAATAGCTGAAGAGCCATCTGAGCTGTTGCTGGTCCAAGGTCGTTGTCCATCATATTGTTCTTTATCACTGTAACACCAATGCTCATGTCAGCCGATTCATTAATCCCAGACACTCCA	3948–4086	139	-33.8
4	H5N1V-MD105	Direct	AGATGGCTCATTGCTGAATGTAGAAATATACTGACAAAGACTGAAAATAGCTTTGAACAGATAACATTTTTGCAAGCATTGCAACTCT	13735–13822	84	-17.0
5	H5N1V-MD76	Direct	TCGTGCCTTCCTTTGACATGAGTAATGAAGGATCTTATTTCTTCGGAGACAATGCAGAGGAATATGA	10342–10390	67	-20.7
6	H5N1V-MR90	Reverse	TAGCATTCTGGCGTTCTCCACCAGTTTCCATCTGTTCATAAGATCGTTTGGTGCCCTGAGACGCCATGATGTTG	8894–8967	74	-26.6
7	H5N1V-MD94	Direct	AGTGGCTTTTGGCCTAGTGTGTGCCACTTGTGAGCAGATTGCAGATTCACAGCATCGGTCTCACAGACAGATGGCAACTACCACCAACCCACT	12405–12497	93	-28.5
8	H5N1V-MR64	Reverse	GGATCCAGCTTGCTAGTGATCTAGGCTTTGGTTCATCACTGTCATACTGCCTTAGATCGCTAACATCTTTGCAATCC	5946–6022	77	-21.4
9	H5N1V-MD101	Direct	AGCTACTCAGTATGAGAGACATGTGTGATGCCCCCTTTGATGACAGGCTCCGAAGAGACCAAAAGGCATTAAAGGGAAGAGGCAGCACACTTGGACTCGATTTAAGAGTGGCT	13093–13205	113	-45.6