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The tissue was collected at the time of euthanasia. Hematoxylin and eosin (H&E) staining was performed to visualize histopathology. The presence of EEEV RNA and proteins was visualized via in situ hybridization (ISH) and immunohistochemistry (IHC), respectively. H&E, ISH, and IHC were performed on the tissues of all four NHPs. Bar = 100 um (H&E and IHC). Bar = 50 um (ISH).
