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. Author manuscript; available in PMC: 2022 May 9.
Published in final edited form as: Neuron. 2016 Jun 9;91(1):119–132. doi: 10.1016/j.neuron.2016.05.016

Figure 5. Requirement of Oligodendroglial NMDA Receptors for High-Frequency Conduction.

Figure 5.

(A) Scheme of stimulating DRG axons of lumbar segment L4 and recording from fasciculus gracilis at L1 (inset: intact myelination of spinal cord in mutants and controls, Gallya’s stain).

(B) Representative CAPs (averaged) in control (top) and mutants at age 4–6 months. Dotted line: peak-to-peak amplitude used to analyze firing strength of fastest axon groups. Conduction delays were unchanged.

(C) Normal axonal excitability in mutants measured at increasing stimulus intensities with peak-to-peak amplitude normalized to maximal readings.

(D) After 10 min at 0.1 Hz, stimulation frequency increased to 10 Hz (medium frequency stimulation, MFS), showing no difference between mutants (n = 6) and controls (n = 3).

(E) 100 Hz (high frequency stimulation, HFS) caused a decrease of firing strength (within seconds) that was significantly faster and stronger in NR1 mutants (n = 7) than controls (n = 5). Left inset: higher temporal resolution for indicated region, Finteraction (119, 1,190) = 3.07, p < 0.0001, two-way ANOVA). Right inset: slower recovery of CAPs at the end of HFS, monitored at 0.1 Hz (τ calculated from normalized exponential fits, p = 0.0084, Student’s t test).