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. 2022 Mar 8;11(3):1349–1360. doi: 10.1021/acssynbio.1c00631

Figure 3.

Figure 3

Engineering of E. coli mini-SimCells for specific cancer cells adhesion. (a) (i) Pre- and (ii) post-purification of pNVC17_sfGFP transformed E. coli mini-SimCells (green). Scale bar is 10 μm. (b) Two-hour incubation of pNVC17_sfGFP or pNVCYT_sfGFP transformed mini-SimCells with high CEA-expressing Caco2 and low CEA-expressing SW480. The red box shows the zoom-in region using 20× magnification. Nuclei were stained with Hoechst dye (blue). Microscopic images at different time points throughout the 8-h incubation can be found in Supporting Information Figure S5. Scale bars are 100 and 10 μm for the zoom-in region.