Table 7.

Columns contained in scored data output from the combinatorial-targeting scoring mode obtained in Procedure 2, Steps 15 and 19, and Procedure 3, Step 4. SCREEN placeholders represent the names of all scored screens.

Scored data column	Description
gene1	Gene symbol targeted by the first guide
gene2	Gene symbol targeted by the second guide
n_combn_[SCREEN]	Number of combinatorial-targeting guides post-filtering for the screen
n_single_[SCREEN]	Number of single-targeting guides post-filtering for the screen
mean_combn_[SCREEN]	Mean LFC across all combinatorial-targeting guides and both orientations
mean_single_[SCREEN]	Mean LFC across all combinations of single-targeting LFC sums that match combinatorial-targeting guide IDs, for both orientations
var_combn_[SCREEN]	Variance for all combinatorial-targeting guides across both orientations
var_single_[SCREEN]	Variance for all combinations of single-targeting LFC sums across both orientations
orientation_agree_[SCREEN]	True if both orientations’ differential effects have the same sign, false otherwise.
differential_combn_vs_single_[SCREEN]	Loess-adjusted (if specified) differential between mean combn and single LFCs
pval1_combn_vs_single_[SCREEN]	P-value between loess-adjusted residuals for the first orientation of combn and single LFCs
pval2_combn_vs_single_[SCREEN]	P-value between loess-adjusted residuals for the second orientation of combn and single LFCs
fdr1_combn_vs_single_[SCREEN]	FDR-adjusted p-value for the first orientation
fdr2_combn_vs_single_[SCREEN]	FDR-adjusted p-value for the second orientation
significant_[SCREEN]	Significance calls returned from call_significant_response_combn
effect_type_[SCREEN]	Effect type calls returned from call_significant_response_combn