TABLE 1.
ELISA RT-PCR detection of transient infection of A431 cultures by HPV-11 and HPV-40
| Virus | Concn of PCR productsa | Mean (SD) of ELISA OD readings
|
|
|---|---|---|---|
| HPV-11 probe | HPV-40 probe | ||
| HPV-11 | 10 | 1.827 (0.174) | −0.013 (0.000) |
| 1 | 1.540 (0.034) | NTb | |
| 0.1 | 0.845 (0.039) | NT | |
| HPV-40 | 10 | 0.012 (0.002) | 2.000 (0.000) |
| 1 | NT | 1.842 (0.080) | |
| 0.1 | NT | 1.027 (0.028) | |
Volume (in microliters) of reaction products from the second set of PCR amplification products added to the ELISA wells.
NT, not tested.