TABLE 2.
RT-PCR ELISA for detection of transient infection of human A431 cells with HPV-11 or HPV-40a
| Expt and treatment (μg/ml)b | Mean (SD) of OD reading for RT-PCR ELISA
|
|
|---|---|---|
| HPV probe | β-Actin probe | |
| Expt 1 | ||
| Cells alone | 0.043 (0.004) | 1.645 (0.052) |
| HPV-11 only | 1.417 (0.063) | 1.564 (0.051) |
| Without virus | ||
| CS (1,000) | 0.028 (0.004) | 1.427 (0.013) |
| DS (1,000) | 0.000 (0.000) | 1.470 (0.001) |
| PSS (1,000) | 0.038 (0.001) | 1.467 (0.158) |
| With virus | ||
| CS (1,000) | 0.038 (0.001) | 1.518 (0.020) |
| CS (100) | 0.049 (0.001) | 1.532 (0.025) |
| CS (10) | 1.485 (0.045) | 1.576 (0.021) |
| DS (100) | 0.035 (0.000) | 1.583 (0.022) |
| DS (10) | 0.023 (0.000) | 1.636 (0.105) |
| PSS (1,000) | 0.045 (0.001) | 1.565 (0.040) |
| PSS (100) | 0.036 (0.002) | 1.585 (0.022) |
| PSS (10) | 0.011 (0.004) | 1.629 (0.057) |
| Expt 2 | ||
| With virus | ||
| PSS (100) | 0.008 (0.006) | 1.002 (0.186) |
| PSS (10) | 1.045 (0.016) | 1.094 (0.074) |
| PSS (1) | 0.893 (0.100) | 0.145 (0.015) |
| PSS (0.1) | 0.714 (0.026) | 1.171 (0.042) |
| Expt 3 | ||
| With virus | ||
| CS (1,000) | 0.037 (0.004) | NDc |
| CS (100) | 0.128 (0.008) | ND |
| CS (10) | 0.397 (0.050) | ND |
| DS (1,000) | 0.006 (0.007) | ND |
| DS (100) | 0.375 (0.073) | ND |
| DS (10) | 0.042 (0.007) | ND |
| PSS (1,000) | 0.002 (0.003) | ND |
| PSS (100) | 0.732 (0.047) | ND |
| PSS (10) | 1.729 (0.384) | ND |
| HPV-40 only | 1.320 (0.074) | ND |
a
Human A431 cells were infected with HPV-11 (experiments 1 and 2) or HPV-40 (experiment 3), and HPV-11 probe (experiments 1 and 2) or HPV-40 probe (experiment 3) was used. Two additional experiments yielded similar results.
b
Concentrations shown are the viral pretreatment doses for CS, DS, and PSS.
c
ND, not determined.