| Strain, strain background (Rattus norvegicus) |
Long–Evans |
Charles River Laboratories |
Strain: 006; RRID:RGD_2308852
|
|
| Strain, strain background (Mus musculus) |
C57BL/6 |
Charles River Laboratories |
Strain:027; RRID:IMSR_CRL:27
|
|
| Cell line |
HEK293T |
ATCC |
CRL-3216; RRID:CVCL_0063
|
|
| Antibody |
Anti-Shank3(guinea pig polyclonal) |
Synaptic Systems |
Cat# 162304; RRID:AB_2619863
|
IF (1:1000); WB (1:1000) |
| Antibody |
Anti-pS1615(rabbit polyclonal) |
This paper |
|
WB (1:1000)Detailed information can be found in section ‘Generation and validation of phosphospecific Shank3 antibody’, and the reagent is available upon request |
| Antibody |
Anti-HA(rabbit monoclonal, C29F4) |
Cell Signaling Technology |
Cat# 3724S; RRID:AB_1549585
|
IP (1:500) |
| Antibody |
Anti-HA(chicken polyclonal) |
Aves Labs |
Cat# ET-HA100; RRID:AB_2313511
|
WB (1:1000) |
| Antibody |
Anti-rabbit Alexa Fluor 680(donkey polyclonal) |
Jackson ImmunoResearch |
Cat# 711-625-152; RRID:AB_2340627
|
WB (1:5000) |
| Antibody |
Anti-guinea pig IRDye 800CW(donkey polyclonal) |
LI-COR |
Cat# 925-32411; RRID:AB_2814905
|
WB (1:5000) |
| Antibody |
Anti-chicken IRDye 800CW(donkey polyclonal) |
LI-COR |
Cat# 925-32218; RRID:AB_2814922
|
WB(1:5000) |
| Antibody |
Anti-GFP(chicken polyclonal) |
Aves Labs |
Cat# GFP-1020; RRID:AB_2307313
|
IF (1:1000) |
| Antibody |
Anti-VGluT1(guinea pig polyclonal) |
Synaptic Systems |
Cat# 135304; RRID:AB_887878
|
IF (1:1000) |
| Antibody |
Anti-VGluT1(rabbit polyclonal) |
Synaptic Systems |
Cat# 135302; RRID:AB_887877
|
IF (1:1000) |
| Antibody |
Anti-GluA2(mouse monoclonal) |
Gift from Eric Gouaux, OHSU |
|
IF (1:1000) |
| Antibody |
Anti-chicken Alexa 488(goat polyclonal) |
Thermo Fisher Scientific |
Cat# A-11039; RRID:AB_142924
|
IF (1:400) |
| Antibody |
Anti-mouse Alexa 555(goat polyclonal) |
Thermo FisherScientific |
Cat# A-21424; RRID:AB_141780
|
IF (1:400) |
| Antibody |
Anti-guinea pig Alexa 555(goat polyclonal) |
Thermo FisherScientific |
Cat# A-21435; RRID:AB_2535856
|
IF (1:400) |
| Antibody |
Anti-guinea pig Alexa 647(goat polyclonal) |
Thermo FisherScientific |
Cat# A-21450; RRID:AB_2735091
|
IF (1:400) |
| Antibody |
Anti-rabbit Alexa 647(goat polyclonal) |
Thermo FisherScientific |
Cat# A-21245; RRID:AB_2535813
|
IF (1:400) |
| Recombinant DNA reagent |
Shank3 Short Hairpin (PVLTHM) |
Gift from Chiara Verpelli |
|
Verpelli et al., 2011
|
| Recombinant DNA reagent |
pDEST53-CMV-Cycle3GFP-Shank3 WT (short-hairpin insensitive) |
Gift from Chiara Verpelli |
|
Verpelli et al., 2011
|
| Recombinant DNA reagent |
pDEST53-CMV-Cycle3GFP-Shank3 S1586A/ S1615A |
This paper |
|
Detailed information can be found in the section ‘Expression constructs and generation of Shank3 mutants,’ and the reagent is available upon request |
| Recombinant DNA reagent |
pDEST53-CMV-Cycle3GFP-Shank3 S1586D/ S1615D |
This paper |
|
Detailed information can be found in the section ‘Expression constructs and generation of Shank3 mutants,’ and the reagent is available upon request |
| Recombinant DNA reagent |
pDEST53-CMV-HA-Shank3 WT |
This paper |
|
Detailed information can be found in the section ‘Expression constructs and generation of Shank3 mutants,’ and the reagent is available upon request |
| RecombinantDNA reagent |
pDEST53-CMV-HA-Shank3 S1586A/ S1615A |
This paper |
|
Detailed information can be found in the section ‘Expression constructs and generation of Shank3 mutants,’ and the reagent is available upon request |
| Recombinant DNA reagent |
pAAV-CMV-PI-EGFP-WPRE-bGH |
Gift from James M. Wilson |
Addgene# 105530; RRID:Addgene_105530
|
|
| Commercial assay or kit |
Lipofectamine 2000 |
Thermo FisherScientific |
Cat# 11668-027 |
|
| Commercial assay or kit |
Gibson Assembly Master Mix |
New England Biolabs |
Cat# E2611S |
|
| Commercial assay or kit |
Lambda protein phosphatase |
New England Biolabs |
Cat# P0753S |
|
| Commercial assay or kit |
BCA Protein Assay Kit |
Thermo FisherScientific |
Cat# 23227 |
|
| Commercial assay or kit |
Protein-G Magnetic Beads |
Thermo FisherScientific |
Cat# 88847 |
|
| Commercial assay or kit |
SimplyBlue SafeStain |
Thermo FisherScientific |
Cat# LC6060 |
|
| Commercial assay or kit |
PP2A Immunoprecipitation Phosphatase Assay Kit |
Millipore |
Cat# 17-313 |
|
| Commercial assay or kit |
Ni-NTA Superflow Agarose Beads |
QIAGEN |
Cat# 30410 |
|
| Chemical compound, drug |
Tetrodotoxin |
Tocris |
Cat# 1069 |
|
| Chemical compound, drug |
Bicuculline methobromide |
Tocris |
Cat# 0109 |
|
| Chemical compound, drug |
Picrotoxin |
Sigma-Aldrich |
Cat# P1675 |
|
| Chemical compound, drug |
Okadaic acid |
Santa Cruz |
Cat# sc-3513 |
|
| Chemical compound, drug |
Tautomycetin |
Tocris |
Cat# 2305 |
|
| Chemical compound, drug |
Fostriecin |
Tocris |
Cat# 1840 |
|
| Chemical compound, drug |
KN62 |
Tocris |
Cat# 1277 |
|
| Chemical compound, drug |
KN93 |
Tocris |
Cat# 1278 |
|
| Chemical compound, drug |
H89 |
Tocris |
Cat# 2910 |
|
| Chemical compound, drug |
Sequencing-grade trypsin |
Promega |
Cat# V5111 |
|
| Chemical compound, drug |
Tandem Mass Tag (TMT) 10plex |
Thermo FisherScientific |
Cat# 90110 |
|
| Software, algorithm |
Image Lab Software |
Bio-Rad |
RRID:SCR_014210
|
https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z&source_wt=imagelabsoftware_surl
|
| Software, algorithm |
ZEN Black |
Zeiss |
RRID:SCR_018163
|
https://www.zeiss.com
|
| Software, algorithm |
Metamorph |
Molecular Devices |
RRID:SCR_002368
|
http://www.moleculardevices.com/Products/Software/Meta-Imaging-Series/MetaMorph.html
|
| Software, algorithm |
Fiji |
Fiji |
RRID:SCR_002285
|
http://fiji.sc
|
| Software, algorithm |
GraphPad Prism |
GraphPad |
RRID:SCR_002798
|
http://www.graphpad.com/
|
| Software, algorithm |
IGOR pro |
Wavemetrics |
RRID:SCR_000325
|
https://www.wavemetrics.com/products/igorpro/igorpro.htm
|
| Software, algorithm |
Spectrum mill v.7.00.208 |
Agilent Technologies |
|
|
| Software, algorithm |
R v 4.0 |
The R Foundation |
RRID:SCR_001905
|
https://www.R-project.org/
|
| Software, algorithm |
Cytoscape v 3.8.2 |
Cytoscape |
RRID:SCR_003032
|
http://cytoscape.org
|
| Software, algorithm |
EnrichmentMap |
EnrichmentMap |
RRID:SCR_016052
|
http://baderlab.org/Software/EnrichmentMap
|
| Other |
Odyssey Blocking Buffer |
LI-COR |
Cat# 927-40000 |
Detailed information can be found in the section ‘Immunoprecipitation and Western blotting’
|
| Other |
DAPI-Fluoromount-G |
SouthernBiotech |
Cat# 0100-20 |
Detailed information can be found in the section ‘Immunocytochemistry’ |
