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High glucose exposure dose-dependently upregulated USF2 protein levels in rat mesangial cells. Primary rat mesangial cells (p4) were cultured and made quiescent. Cells were then treated with different concentrations of d-glucose media for 24 h. USF2 protein levels in nuclear extracts were determined by Western blotting. Relative USF2 levels were determined by scanning densitometry of immunoblots and normalized to β-actin levels. Results are the mean ±SE (n = 3). *P < 0.05 versus mM.
