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. Author manuscript; available in PMC: 2022 May 9.
Published in final edited form as: J Cell Biochem. 2008 Apr 15;103(6):1952–1961. doi: 10.1002/jcb.21585

Fig. 3.

Fig. 3.

High glucose exposure had no effect on USF2 protein stability. Quiescent rat mesangial cells were treated with 5 mM (NG) (A) or 30 mM (HG) (B) glucose media for 24 h. Cells were then treated with cycloheximide (10 μg/ml) for indicated time period. After treatment, nuclear extracts were prepared and USF2 protein levels were determined by Western blotting. (C) Relative USF2 levels were determined by scanning densitometry of immunoblots and normalized to β-actin levels. Measurement of the ratio of USF2/actin at time = 0 was assigned a relative value of 100%. The results are expressed as mean ±SE of three separate experiments.