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High glucose exposure time-dependently stimulated USF2 mRNA levels in rat mesangial cells. Quiescent rat mesangial cells were treated normal (5 mM) or high glucose (30 mM) media for indicated time points. After treatment, cells were harvested and total RNA was extracted. USF2 mRNA levels were determined by real-time PCR as described in Materials and Methods. The results are expressed as mean ±SE of three separate experiments. *P < 0.05 versus NG.
