Fig. 5.

High glucose exposure had no effect on USF2 mRNA stability. Quiescent rat mesangial cells were treated with actinomycin D (5 μg/ml) in the presence of normal (5 mM) or high glucose (30 mM) media for indicated period. After treatment, cells were harvested and total RNA was extracted. Northern analysis and hybridization for USF2 and β-actin were performed as described in Materials and Methods to measure the rate of decay of USF2 mRNA and the half-life. Measurement of the ratio of USF2/actin at time = 0 was assigned a relative value of 100%. The results are expressed as mean ±SE of three separate experiments.