Fig. 8.

Chromatin immunoprecipitation analysis demonstrated the interaction of CREB with the USF2 promoter. A: Schematic representation showing CREB binding site. Two arrows indicate primers used for amplifying the region from −1,742 to −1,620 spanning the CRE site. B: Formaldehyde-cross-linked chromatin isolated from primary mesangial cells was immunoprecipitated (IP) with anti-CREB antibody and subjected to PCR as described under “Materials and Methods.” PCR products were electrophoresed on 2% agarose gel. The experiments were repeated for three times, and the representative result is shown.