Figure 6.

The PI3K/Akt signaling pathway is involved in endothelin-1 or ET-1–mediated NFκB activity IL-6 and TNF-α expression. (A–E) C2C12 and G7 cells were pretreated with BQ123, BQ788, Ly294002, or Akt inhibitor followed, by stimulation with ET-1, and were analyzed using immunofluorescence assay. (F) C2C12 and G7cells were incubated for 24 h with various concentrations of ET-1, and in vitro NFκB-luciferase activity was measured. (G) C2C12 and G7cells were pretreated with BQ123, BQ788, Ly294002, or Akt inhibitor for 30 min stimulated for 24 h with ET-1 (50 nM), and in vitro NFκB–luciferase activity was measured. Results are expressed of four independent experiments performed in triplicate. ^*p < 0.05 compared with control; ^#p < 0.05 compared with the ET-1–treated group.