Figure 7.

Endothelin-1 (ET-1) enhances IL-6, TNF-α, and visfatin synthesis and reduces mitochondria oxygen consumption in AT. (A) Bright-field image (100×) of 3T3-L1 preadipocytes cells were incubated with supernatant from C2C12 and observes for 3days with oil-drop. (B) Supernatant from C2C12 and G7 treated with ET-1 were incubated with 3T3-L1 cells. in vitro IL-6, TNF-α, and visfatin activity were measured through Western blotting. (C) 3T3L-1 cell mitochondrial energy measured by Seahorse. Representative OCR plot with the injection of oligomycin, FCCP, and rotenone. (D) Quantization data of the different areas of ORC. All the control groups in Figure 7 are from the control CM of C2C12 without treat with ET-1. Results are expressed of four independent experiments performed in triplicate. ^*p < 0.05 compared with control; ^#p < 0.05 compared with the ET-1–treated group.