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. 2001 Dec;45(12):3456–3461. doi: 10.1128/AAC.45.12.3456-3461.2001

FIG. 2.

FIG. 2

The pH dependency of light production of S. aureus RN4220/pTOO24 and RN4220/pCSS810 cells. The assay was performed in citrate-phosphate buffer (pH range, 4.6 to 5.6 [⧫]), MES (pH range, 5.6 to 6.6 [▪]) and MOPS (pH range, 6.6 to 7.0 [▴]) as described in Materials and Methods. Panel A shows the pH dependency of d-luciferin penetration into the indicator RN4220/pTOO24 cells, and Panel B shows results for strain RN4220/pCSS810.