Table 1.

Detection of SARS-CoV-2 targets at different dilutions tested by RT-ddPCR. Average and range of N1 copies, N2 copies, N1 positive droplets, N2 positive droplets, and total droplets for LOD experiments with known SARS-CoV-2 virion copies, as indicated.

SARS-CoV-2 Copies Input	N1 copies detected /reaction	N2 copies detected /reaction	N1 positive droplets	N2 positive droplets	Total Droplets
80	80.7 (80–82)	91.0 (78–101)	49.7 (39–56)	57.0 (38–71)	14,537 (11519–16535)
40	58.3 (53–66)	63.0 (60–66)	37.7 (34–41)	40.7 (38–43)	15,261 (14656–16092)
20	24.7 (19–31)	20.3 (20–21)	15.3 (11–20)	12.3 (12–13)	14,441 (13507–15383)
10	9.7 (7–16)	12.7 (11–14)	6.0 (4–10)	8 (7–9)	14,653 (14377–15103)
5	9.3 (7–12)	7.0 (5–8)	5.7 (4–7)	4.3 (3–5)	14,059 (12848–15280)
0	0.8 (0–4)	0 (0)	0.4 (0–2)	0 (0)	14,821 (12646–16887)
NTC	0 (0)	0 (0)	0 (0)	0 (0)	16,555 (15492–18412)