Fig. 4. Sequence alignment of UDPGlcNAc glycosyltransferases and mutant activities. (a) Sequence alignment showing serine thought to be involved in O-galactosylation and O-GlcNAcylation. (b) Sequence alignment showing serine in other Group H UGTs, thought to be involved in O-galactosylation and O-GlcNAcylation, (c) mutants' donor activity results: comparison between wild type enzymes 76E5, 76E1 and 76E2 and mutant enzymes 76E5 S311N, 76E1 S318N and 76E2 N320S activity with donor compounds (A) UDPGlc, (B) UDPGal, (C) UDPGlcNAc. 76E2 N320S mutant newly acquired UDPGal and UDPGlcNAc activities.