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| Gene name | Forward primer | Reverse primer | Product size (bp) | Reaction temperature (°C) |
|---|---|---|---|---|
| 16S rDNA | 27F | 1492R | 1450 | 55 |
| AGAGTTTGATCCTGGCTCAG | TACGGTTACCTTGTTACGACTT | |||
| amoA | amoA-1F | amoA-2R | 606 | 55 |
| AAGGATTGGCCATTGCTCTG | GTGGACCTAAAATCCAAGCATT | |||
| 16S rDNA a | GCTAGGTAGCAACCCTTTGTAC | CAGCTCGTGTCGTGAGATGT | 203 | 55 |
| amoA a | AAGTCGTTGCTGCCCATAG | TACCCACAAACAGAGCCAGAC | 168 | 55 |
Primers were used in real-time PCR.
