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. 2022 Apr 25;13:852596. doi: 10.3389/fmicb.2022.852596

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Copyright © 2022 Zhao, Feng and Zong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The fitness conferred by plasmid pKPC-2_Vir. Tx090515vir was J53 AziR transconjugant containing pKPC-2_Vir and Tx01 was a derivate of Tx090515vir with the loss of pKPC-2_Vir during sub-cultivating. 115112 was a ST11 K64 CRKP clinical strain and Tx115112 was a transconjugant containing pKPC-2_Vir of 115112. Bacterial growth measured by OD_{600 nm} at different time points (h) are displayed. Compared with J53 AziR, Tx090515vir had lower growth, while Tx01 displayed a similar growth rate. Compared with 115112, Tx115112 exhibited decreased growth.