TABLE 1.
Gene/sequence | Primers | Sequence(5′– 3′) |
iucA | iuc-F | TTCCGAGTGGTAGTGGTT |
iuc-R | GTGGCTTGAAGAGTGAGAA | |
rmpA | rmpA-F | ATTGGTTGACAGCAGGATT |
rmpA-R | GTTAGCCGTGGATAATGGT | |
bla KPC | KPC-F | ATGTCACTGTATCGCCGTCT |
KPC-R | TTTTCAGAGCCTTACTGCCC | |
repFIB K | repFIBK_F | CTATCTGGATTACACCGAGTT |
repFIBK__R | CCTTCTCTTCTTCCTCCTTG | |
repFIIpKP91 | repFIIpKP91_F | GTGTCCTGGCTGTATCTG |
repFIIpKP91_R | TGTTGTTCCGCATCAAGT | |
Fusion site 1 | FS_1_F | TTTTCCTGAACACTGCGCCG |
FS_1_R | GTGAAATAGAACAGGTGACC | |
Fusion site 2 | FS_2_F | CATTTTCGGTCCCATTCTGGC |
FS_2_R | GCTACAGGCAGTCTTCGGCG |
All primers are self-designed. However, the primers for blaKPC–2 are identical to those used in a study (Carattoli et al., 2021), which is likely a coincidence. The primers for iucA have been published by us in another study (Zhao et al., 2022).